Coombs sera & potentiators

Reagents for the (in)direct antiglobulin test (Coombs sera)

MAHS

Monospecific anti-human globulin: for the detection of IgG antibodies on human red cells or the detection of red cell antibodies in human serum

K1131

PAHS

Polyspecific anti-human globulin: for the detection of complement components and IgG antibodies on human red cells or the detection of red cell antibodies in human serum

K1194

anti-C3d

Reagents for the detection of complement components on human red cells

K1377

AHG reagents

Anti-human globulin (AHG) contains antibodies against human IgG. These antibodies bind to the IgG-molecules on the erythrocytes. This forms a bridge and the erythrocytes agglutinate. Most allo-antibodies directed against erythrocytes are IgG-antibodies. Polyspecific AHG reagent is mainly used in the antiglobulin test (saline, albumin or LISS). In the PEG and polybrene technique anti-IgG is always used, seeing as this contains only anti-IgG (monospecific anti-human globulin reagent). Anti-complement in these techniques practically always results in false positive results.

Agglutination potentiating reagents

The effect of using agglutination enhancers is to reduce the water mantle and ion cloud around the erythrocyte, or to increase the interaction between antigen and antibody. Agglutination enhancers can influence the zeta potential in such a way that incomplete antibodies can agglutinate red cells and can increase the binding (association) between antibody and antigen. One of the techniques used is the PEG method, which is very sensitive. Here, antibodies that can only be detected in other techniques because of their complement activating capacity (like anti-Jka and anti-Jkb), can be detected in the PEG-IAT without the use of an anti-complement.

BSA 22%

Bovine Serum Albumin

K1106

PeliLISS

A modified low ionic strength solution

K1110

PEG 4000 20%

Polyethylene glycol 4000

K1159