Coombs sera & potentiators
Reagents for the (in)direct antiglobulin test (Coombs sera)
Monospecific anti-human globulin: for the detection of IgG antibodies on human red cells or the detection of red cell antibodies in human serumK1131
Polyspecific anti-human globulin: for the detection of complement components and IgG antibodies on human red cells or the detection of red cell antibodies in human serumK1194
Agglutination potentiating reagents
The effect of using agglutination enhancers is to reduce the water mantle and ion cloud around the erythrocyte, or to increase the interaction between antigen and antibody. Agglutination enhancers can influence the zeta potential in such a way that incomplete antibodies can agglutinate red cells and can increase the binding (association) between antibody and antigen. One of the techniques used is the PEG method, which is very sensitive. Here, antibodies that can only be detected in other techniques because of their complement activating capacity (like anti-Jka and anti-Jkb), can be detected in the PEG-IAT without the use of an anti-complement.