Platelet Crossmatching

Discover MASPAT in just 2 minutes

Platelet refractoriness remains a major challenge in transfusion medicine. Finding compatible platelet donors can be complex, while conventional compatibility testing often requires specialized equipment and lengthy workflows.

In the short video below, discover how MASPAT provides a practical alternative with a 90-minute workflow, no specialized equipment required, and a clear macroscopic read-out.

Whether you are exploring new approaches to platelet compatibility testing or looking for a solution that fits seamlessly into your laboratory workflow, this video provides a concise introduction to MASPAT and its clinical value.

只需 2 分钟，了解 MASPAT

血小板输注无效仍然是输血医学领域的一项重大挑战。寻找相容的血小板供者可能十分复杂，而传统的相容性检测通常需要专用设备，并且检测流程耗时较长。

在这段简短的视频中，您将了解 MASPAT 如何提供一种实用的替代方案：检测流程仅需 90 分钟，无需专用设备，并可通过清晰的肉眼结果进行判读。

无论您是在探索血小板相容性检测的新方法，还是在寻找一种能够无缝融入实验室日常工作流程的解决方案，本视频都将为您简要介绍 MASPAT 及其临床价值。

▶️ 请观看下方视频。

MASPAT is a solid-phase microwell plate system for the detection of platelet antibodies (anti-HLA and anti-HPA) in serum by testing donor platelets with patient serum in a crossmatching assay.

Principle

The principle of the test is the solid phase technique, which is based on antigen/antibody reaction. A monolayer of donor platelets is immobilised by centrifugation onto the surface of the microplate wells coated with platelet-specific mouse monoclonal antibody.

Patient serum and LISS (Low Ionic Strength Solution) are incubated in the appropriate wells, allowing serum antibodies to bind to the immobilised platelet monolayer. After incubation, non-bound serum components are removed by washing. Platelet-bound antibodies are detected by the addition of mouse monoclonal anti-human IgG and human IgG-sensitised erythrocytes (MASPAT Indicator Red Cells) and subsequent centrifugation of the microplate.

Test results

In case of a positive reaction the anti-human IgG and MASPAT Indicator Red Cells bind to the IgG-antibodies on the platelet monolayer. Positive reactions are thus characterised by adherence of indicator red blood cells throughout the surface of the wells, whereas negative reactions produce discrete pellets of Indicator Red Cells in the middle of the well.

Experience

It is the general experience that in a significant proportion of HLA-matched platelet transfusions administered to allo-immunised patients a satisfying rise in platelet count remains absent. This is caused by platelet specific antibodies (HPA).