Kits
For Monocyte Activation Test
Essange Reagents & Sanquin’s MAT kit: A highly sensitive in vitro human cell-based assay for pyrogen detection, worldwide available through our partner Lonza
The European Pharmacopeia (EP) states that, after a product-specific validation, the Monocyte Activation Test (MAT) is suitable as a replacement for the rabbit pyrogen test.
The MAT Cell Set offers a pool of cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) with dedicated Culture Medium Supplement ensuring a high sensitivity.
Test procedure
Day 1:
Under non-pyrogenic conditions:
- To a 96-wells plate add culture medium, endotoxin standard, product samples & MAT Cells
- Incubate for 18-24h at 37ºC
Day 2 (ELISA):
Under standard lab conditions:
- Transfer supernatant into cytokine ELISA
- Measure cytokine levels and analyze samples
Essange Reagents products for MAT
Day 1 (Cell incubation):
- 3 vials of cryopreserved pooled PBMCs
- 3 vials of MAT Human Serum Supplement
- Fully compliant with Ph. Eur. chapter 2.6.30. requirements
- Limit of Detection (LOD) for endotoxin ≤ 0.08 EU/mL*
- Improved detection of Non-Endotoxin Pyrogens
- 3 vials of cryopreserved pooled PBMCs
- 3 vials of dedicated MAT Culture Medium Supplement (based on FBS)
- PMBCs qualified according to Ph. Eur. chapter 2.6.30
- Limit of Detection (LOD) for endoxtoxin ≤ 0.02 EU/mL
- Detection of Non-Endotoxin Pyrogens
Day 2 (ELISA):
PeliKine Human IL-6 ELISA Rapid Set A (M2018) [NEW]
PeliKine Human IL-6 ELISA Rapid Set B (M2019) [NEW]
- Highly sensitive IL-6 ELISA sufficient for three 96-well plates
- IL-6 standard range (0.6 – 450 pg/mL)
- Limit of Detection (LOD): 1 pg/mL (static incubation)
Why use Essange Reagents MAT kit?
- Qualified according to European Pharmacopoeia Chapter 2.6.30
- High sensitivity
- Reactive towards non-endotoxin pyrogens
- Measurement of IL-6 as cytokine read-out
- Proven robustness and reliability
- Storage at -80ºC (no liquid nitrogen required)
- Assay time 24 hours including overnight culture
- MAT cell Set HS (M2017) further reduces the use of animal-derived components
Why cryopreserved pooled PBMC as cell source?
- Comparable reactivity to fresh PBMC
- No need for blood donors and cell isolation for each experiment
- Pool of 4 donors taking donor variation into account
- Available on demand
- Storage at -80ºC (no liquid nitrogen required)
- Proven robustness and reliability
Why IL-6 as cytokine read-out?
- High sensitivity
- Fully secreted into the medium
- Stable in (frozen) supernatant
Interested? Please mail to info@essange-reagents.nl
*Concentration stated as measured in the sample.